Journal: The Journal of Veterinary Medical Science

Article Title: Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles

doi: 10.1292/jvms.14-0617

Figure Lengend Snippet: Immunohistochemical localization of inhibin α and betaglycan in porcine follicles (original magnification × 100). Brown indicates the presence of the specified protein. (A–C) Normal large follicles: anti-inhibin α IgG (A), anti-betaglycan IgG (B) and negative control (C). (D–F) Cystic follicles: anti-inhibin α IgG (D), anti-betaglycan IgG (E) and negative control (F). G indicates granulosa cells, and T indicates theca cells.

Article Snippet: Then, the sections were incubated overnight at 4°C with rabbit anti-mouse polyclonal antibodies directed against inhibin α or betaglycan (Bioss, Beijing, P.R.

Techniques: Immunohistochemical staining, Negative Control

Journal: The Journal of Veterinary Medical Science

Article Title: Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles

doi: 10.1292/jvms.14-0617

Figure Lengend Snippet: Analysis of INHA and betaglycan mRNA in cystic follicles (grey) and normal large follicles (dark). The relative mRNA levels of INHA and betaglycan presented in the figure were corrected to the level of β-actin gene mRNA. Bars indicate the mean ± SEM inhibin α and betaglycan intensities relative to β-actin protein. Different letters above bars indicate statistically significant differences ( P <0.05).

Article Snippet: Then, the sections were incubated overnight at 4°C with rabbit anti-mouse polyclonal antibodies directed against inhibin α or betaglycan (Bioss, Beijing, P.R.

Techniques:

Journal: The Journal of Veterinary Medical Science

Article Title: Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles

doi: 10.1292/jvms.14-0617

Figure Lengend Snippet: Effect of FSH on expression of INHA in granulosa cells in vitro . Relative folds of inhibin α mRNA (A) and protein (B) in cultured granulosa cells after 0 hr, 24 hr and 48 hr treatments with 30 n g/m l FSH. Bars indicate the mean ± SEM. Different letters above bars indicate statistically significant differences ( P <0.05).

Article Snippet: Then, the sections were incubated overnight at 4°C with rabbit anti-mouse polyclonal antibodies directed against inhibin α or betaglycan (Bioss, Beijing, P.R.

Techniques: Expressing, In Vitro, Cell Culture

Journal: The Journal of Veterinary Medical Science

Article Title: Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles

doi: 10.1292/jvms.14-0617

Figure Lengend Snippet: Detection of INHA and betaglycan in cystic follicles and normal large follicles. (A) Representative photographs of Western blotting for inhibin α, betaglycan and β-actin (as an internal control). (B) Western blotting analysis showing inhibin α and betaglycan compared with β-actin protein. Bars indicate the mean±SEM inhibin α and betaglycan intensities relative to β-actin protein. Different letters above bars indicate statistically significant differences ( P <0.05).

Article Snippet: Then, the sections were incubated overnight at 4°C with rabbit anti-mouse polyclonal antibodies directed against inhibin α or betaglycan (Bioss, Beijing, P.R.

Techniques: Western Blot

Journal: The Journal of Veterinary Medical Science

Article Title: Downregulation of the expression of inhibin α subunit and betaglycan in porcine cystic follicles

doi: 10.1292/jvms.14-0617

Figure Lengend Snippet: Hypothesis concerning cystic follicle (CF) formation in the pig. Ovarian development and ovulation are mainly regulated by gonadotrophins via an endocrine pathway. Firstly, FSH stimulates expression of inhibin α and betaglycan in granulosa cells in the ovary, which increases the synthesis of estrogen through upregulation of 3β-HSD. Estrogen leads to LH-induced ovulation through increased expression of LH receptor (A). However, if synthesis of FSH is insufficient in the pituitary, ovulation could fail, and cystic follicles could form due to decreased expression of inhibin α and betaglycan (B).

Article Snippet: Then, the sections were incubated overnight at 4°C with rabbit anti-mouse polyclonal antibodies directed against inhibin α or betaglycan (Bioss, Beijing, P.R.

Techniques: Expressing